The roughly 700 troops will be deployed on various roads in Washington.
The US trucker convoys aim to replicate recent trucker protests in Canada, which paralysed the capital Ottawa.
The National Guard troops will "provide support at designated traffic posts, provide command and control, and cover sustainment requirements" from Saturday until 7 March, a statement said.
Some 50 "large tactical vehicles" will be stationed at traffic posts 24 hours a day, the statement said. The troops will not carry firearms or take part in law enforcement.